Coding
BcsZ

Part:BBa_K2559000:Design

Designed by: Yinpin Huang   Group: iGEM18_SCAU-China   (2018-10-07)


Bacterial cellulose synthase Z (BcsZ) an endoglucanase related to cellulose hydrolysis.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 252


Design Notes

The sequence of BcsZ gene has a PstI restriction endonuclease site,and we have did the base substitution for it.So it is BioBrick RFC10 compatible


Source

It comes from Acetobacter xylinus cgmcc15237

References

https://www.sciencedirect.com/science/article/pii/S0966842X15001201#tbl0005